Grant 41


Research Institution: The Hebrew University of Jerusalem

Principle Investigator:

Assistant Professor Nir Kalisman

Type: International

Project title: Characterisation of DUX 4 protein – protein interactions in FSHD cell lines and tissue biopsies by cross-linking and mass-spectrometry

Status: Active

Summary

DUX4 is the main protein implicated in FSHD, but relatively little is known about its interactions with other proteins. We must learn more of these interactions for rational design of future FSHD therapies that aim to disrupt them. We propose to use a powerful experimental technique – cross-linking and mass-spectrometry – to chart these interactions in unprecedented detail. The great sensitivity of this approach will allow to verify our findings directly in human muscle tissues.